《植物生理学报》 2013, 49(3): 253-258
通信作者:张;E-mail: zy021208@163.com;Tel: 0451-82456373
摘 要:
金属硫蛋白(MT)是一类广泛存在于生物中的低分子量、富含半胱氨酸且可结合重金属的蛋白质, 在土壤重金属污染的治理中发挥重要作用。将从柽柳和星星草cDNA文库中克隆的MT基因构建在同一植物表达载体pRCXM上, 通过农杆菌介导的叶盘法转化烟草‘龙江911’, 获得了抗卡那霉素的转基因植株。PCR-Southern和Northern blot检测证明双价MT基因已整合进烟草基因组中并可正常表达。抗性试验证明双价MT基因的转入提高了转基因烟草对Cd2+和Cu2+的抗性, 转基因烟草分别能在300 μmol·L-1 Cd2+或150 μmol·L-1 Cu2+的胁迫下正常生长。本研究结果对通过基因工程技术培育植物修复新品种提供了理论基础。关键词:金属硫蛋白; 星星草; 柽柳; 烟草
收稿:2012-12-14 修定:2013-01-28
资助:国家“973”计划项目(G1999016000)。
Corresponding author: ZHANG Yan; E-mail: zy021208@163.com; Tel: 0451-82456373
Abstract:
Metallothionein (MT) is a low molecular weight, cysteine-rich, and heavy metal-binding protein. MT plays important roles in controlling heavy metal pollution of soil. Two MT genes from Tamarix androssowii and Puccinellia tenuiflora were cloned into the same vector pRCXM. Kanamycin-resistant transgenic tobacco (Nico-tiana tabacum ‘Longjiang 911’) plants were obtained using Agrobacterium-mediated gene transferring method. PCR-Southern and Northern blot analysis indicated that two MT genes were indeed integrated into the tobacco genome and expressed in tobacco. Resistance tests proved that the expression of two MT genes enhanced toler-ance to Cd2+ and Cu2+ in tobacco. The transgenic plants can live in the medium with 300 µmol·L-1 Cd2+ or 150 µmol·L-1 Cu2+. Our study provides theoretical references for cultivating new varieties of phytoremediation by genetic engineering technology.Key words: metallothionein; Puccinellia tenuiflora; Tamarix androssowii; tobacco (Nicotiana tabacum)
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